Analysis Note

ControlPositive Control: Kidney, muscle, tendon spleen tissue, mouse liver. Negative Control: Neurons/glia.

Application

This Anti-Collagen Antibody, Type IV is validated for use in ELISA, IF, IH(P) for the detection of Collagen.

ELISA:
A previous lot of this antibody was used in ELISA at >1:200 (OD >500).

Immunofluorescence:
A previous lot of this antibody was used in immunofluorescence.

Immunohistochemistry:
1:80 dilution for immunofluorescent staining of fresh frozen mouse skin and liver tissues. Acetone or methyl-carnoy fixed paraffin-embedded tissue (mouse skin and liver) is also reactive.

Not recommended for Western blots.

Optimal working dilutions must be determined by end user.

Research CategoryCell Structure

Research Sub CategoryECM Proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Collagen is the main protein of connective tissue in animals and the most abundant protein in mammals, making up about 25% of the total protein content. It is one of the long, fibrous structural proteins whose functions are quite different from those of globular proteins such as enzymes; tough bundles of collagen called collagen fibers are a major component of the extracellular matrix that supports most tissues and gives cells structure from the outside, but collagen is also found inside certain cells. Collagen has great tensile strength, and is the main component of fascia, cartilage, ligaments, tendons, bone and teeth. Along with soft keratin, it is responsible for skin strength and elasticity, and its degradation leads to wrinkles that accompany aging. It strengthens blood vessels and plays a role in tissue development. It is present in the cornea and lens of the eye in crystalline form. Collagen occurs in many places throughout the body. There are 12 types of collagen described in literature. Collagen Type 1: This is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is found in tendons, the endomysium of myofibrils and the organic part of bone. Collagen Type 4: It is present in basal lamina and the eye lens. Also serves as part of the filtration system in capillaries and the glomeruli of nephron in the kidney.

Collagen Type IV extracted and purified from mouse tumor tissues. Antibody shows less than 0.1% reactivity with human Collagen types IV, V and mouse Collagen Types I, II, III and mouse Fibronectin and Laminin.

Immunogen

Collagen Type IV extracted and purified from mouse tumor tissues.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB756

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

The purified antibody is supplied in a buffer containing a mixture of 0.1M Citrate, 0.1M potassium phosphate, at a pH of 7.2-7.4 and 10µg/ml of antibiotics and antimytotics

Specificity

Mouse Collagen, Type IV: 100 (%'s at a 1:5000 RIA dilution) Mouse Collagen, Types I, II, III: ﹤0.1 Human Collagen, Types IV, V: ﹤0.1 Mouse Fibronectin: ﹤0.1 Mouse Laminin: ﹤0.1 Reactivity with other species has not been determined.

Antibody shows less than 0.1% reactivity with human Collagen types IV, V and mouse Collagen Types I, II, III, mouse Fibronectin, and mouse laminin.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Target description

139 kDa (unprocessed precursor)